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Human CellExp IL-12, Mouse recombinant protein

NKSF, CLMF

     
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Product info
Primary Accession P43432
Calculated MW 70 kDa
Additional Info
Gene ID 16160
Gene Symbol IL12
Other Names NKSF, CLMF, Cytotoxic lymphocyte maturation factor 40 kDa subunit
Gene Source Mouse
Source HEK 293 cells
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 HPLC; ≥95%
Recombinant Yes
Results 0.1 ng/mL
Sequence p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Application Notes Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Format Lyophilized protein
Storage -20°C; Recombinant mouse IL-12 is lyophilized from 0.5 x PBS, pH 7.5.
Citations (0)

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Background

Interleukin-12 (IL-12) is an important mediator of cellular-immunity produced by dendritic cells, macrophages and B cells when stimulated with antigen. IL-12 is involved in differentiating precursor helper T cells to Th1 and/or Th2 subtypes and promotes the production of IFN gamma and TNF alpha. Mouse IL-12 is active on human and mouse cells. Recombinant mouse IL-12 is a glycosylated, disulfide-linked heterodimer. It is containing one p35 subunit (35 kDa) and one p40 subunit (40 kDa), with a total of 506 amino acids and a molecular weight of approximately 70 kDa.

References

Schoenhaut D.S.,et al.J. Immunol. 148:3433-3440(1992).
Tone Y.,et al.Eur. J. Immunol. 26:1222-1227(1996).
Teuscher C.,et al.J. Immunol. 163:2262-2266(1999).
Oppmann B.,et al.Immunity 13:715-725(2000).

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$ 230.00
$ 1,845.00
Cat# PBV10374r
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