Estrogen Sulfotransferase Blocking Peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P49888 |
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Gene ID | 6783 |
Calculated MW | 35126 Da |
Gene ID | 6783 |
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Application & Usage | The peptide is used for blocking the antibody activity of EST. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C. |
Other Names | Estrogen sulfotransferase, 2.8.2.4, EST-1, Sulfotransferase 1E1, ST1E1, Sulfotransferase, estrogen-preferring, SULT1E1, STE |
Target/Specificity | Estrogen Sulfotransferase |
Formulation | 50 µg (0.5 mg/ml) EST peptide in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Estrogen Sulfotransferase Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SULT1E1 |
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Synonyms | STE |
Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone (PubMed:7779757, PubMed:11884392, PubMed:11006110). Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency (PubMed:11006110, PubMed:19589875). Does not sulfonate cortisol, testosterone and dopamine (PubMed:7779757, PubMed:11006110). May play a role in gut microbiota-host metabolic interaction. O-sulfonates 4- ethylphenol (4-EP), a dietary tyrosine-derived metabolite produced by gut bacteria. The product 4-EPS crosses the blood-brain barrier and may negatively regulate oligodendrocyte maturation and myelination, affecting the functional connectivity of different brain regions associated with the limbic system. |
Cellular Location | Cytoplasm, cytosol. |
Tissue Location | Liver, intestine and at lower level in the kidney. |
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