|Calculated MW||35126 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of EST. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Estrogen sulfotransferase, 220.127.116.11, EST-1, Sulfotransferase 1E1, ST1E1, Sulfotransferase, estrogen-preferring, SULT1E1, STE|
|Formulation||50 µg (0.5 mg/ml) EST peptide in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Estrogen Sulfotransferase Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.|
|Tissue Location||Liver, intestine and at lower level in the kidney|
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