|Calculated MW||46844 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Trek-1. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, TREK-1 K(+) channel subunit, Two pore potassium channel TPKC1, Kcnk2|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Trek1 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage- insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2 (PubMed:24496152). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (PubMed:24496152).|
|Cellular Location||Isoform 1: Cell membrane; Multi- pass membrane protein. Note=Location at the cell membrane requires interaction with KCNK1. Is not detected at the cell membrane when KCNK1 is absent.|
|Tissue Location||Detected in hippocampus astrocytes (at protein level) (PubMed:24496152). High expression in brain and lung. Also detected in kidney, heart and skeletal muscle. Not detected in liver. In the brain, highest expression in olfactory bulb, hippocampus and cerebellum.|
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