|Calculated MW||55278 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of DGAT-1. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Diacylglycerol O-acyltransferase 1, 188.8.131.52, ACAT-related gene product 1, Acyl-CoA retinol O-fatty-acyltransferase, ARAT, Retinol O-fatty-acyltransferase, 184.108.40.206, Diglyceride acyltransferase, DGAT1, AGRP1, DGAT|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||DGAT1 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2 it is not essential for survival. May be involved in VLDL (very low density lipoprotein) assembly. In liver, plays a role in esterifying exogenous fatty acids to glycerol. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein|
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