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TFF-3, human recombinant protein
Trefoil factor 3
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q07654 |
|---|---|
| Calculated MW | 13.2 kDa |
| Gene ID | 7033 |
|---|---|
| Gene Symbol | TFF3 |
| Other Names | Trefoil factor 3 (Intestinal trefoil factor) (hITF) (Polypeptide P1.B) (hP1.B) |
| Gene Source | Human |
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥97% |
| Assay2&Purity2 | HPLC; ≥97% |
| Recombinant | Yes |
| Results | 1.0-10.0 ng/ml. |
| Sequence | EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF |
| Application Notes | . Reconstitute in sterile 18 MΩ-cm H₂O at not less than 100 µg/ml. Reconstituted TFF3 should be stored in working aliquots at -20°C. |
| Format | Lyophilized protein |
| Storage | -20°C; Sterile filtered white powder lyophilized from a solution containing PBS pH 7.4 |
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Provided below are standard protocols that you may find useful for product applications.
Background
Proteins of the TFF family are characterized by having at least 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, through an EGFR-dependent pathway.TFF-3 is normally expressed in hepatocellular carcinoma and its overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved disulfide bonds and has MW = 13.2 kDa. TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
References
Hauser F.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:6961-6965(1993).
Seib T.,et al.Genomics 40:200-202(1997).
Berry A.,et al.Genomics 68:22-29(2000).
Hattori M.,et al.Nature 405:311-319(2000).
Casadei R.,et al.Gene 321:185-193(2003).
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