Insig1 Blocking Peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q08755 |
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Other Accession | NP_071787 |
Gene ID | 64194 |
Calculated MW | 28232 Da |
Gene ID | 64194 |
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Application & Usage | The peptide is used for blocking the antibody activity of InSig-1. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C. |
Other Names | Insulin-induced gene 1 protein, INSIG-1, Immediate-early protein CL-6, Insulin-induced growth response protein CL-6, Insig1, Cl-6 |
Target/Specificity | Insig1 |
Formulation | 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Insig1 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Insig1 {ECO:0000312|RGD:708457} |
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Function | Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR. Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. Binds oxysterol, including 25- hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum. In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi. Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG1 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2. Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligases AMFR/gp78 and/or RNF139. Also regulates degradation of SOAT2/ACAT2 when the lipid levels are low: initiates the ubiquitin- mediated degradation of SOAT2/ACAT2 via recruitment of the ubiquitin ligases AMFR/gp78. |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:O15503}; Multi-pass membrane protein {ECO:0000250|UniProtKB:O15503} |
Tissue Location | Highly expressed in liver and kidney. |
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