Furin/PACE Blocking Peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P23188 |
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Other Accession | NP_035176 |
Gene ID | 18550 |
Calculated MW | 86772 Da |
Gene ID | 18550 |
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Application & Usage | The peptide is used for blocking the antibody activity of Furin/PACE. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C. |
Other Names | Furin, 3.4.21.75, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme, PACE, Prohormone convertase 3, Furin, Fur, Pcsk3 |
Target/Specificity | Furin/PACE |
Formulation | 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | Furin/PACE Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Furin |
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Synonyms | Fur, Pcsk3 |
Function | Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed:18713856). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (By similarity). Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-45) (By similarity). By mediating processing of accessory subunit ATP6AP1/Ac45 of the V-ATPase, regulates the acidification of dense-core secretory granules in islets of Langerhans cells (PubMed:18713856). |
Cellular Location | Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:P09958}; Single-pass type I membrane protein. Cell membrane {ECO:0000250|UniProtKB:P09958}; Single- pass type I membrane protein. Secreted {ECO:0000250|UniProtKB:Q28193}. Endosome membrane {ECO:0000250|UniProtKB:P09958}; Single-pass type I membrane protein. Note=Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin {ECO:0000250|UniProtKB:P09958} |
Tissue Location | Seems to be expressed ubiquitously (PubMed:2266110). Expressed in islets of Langerhans (PubMed:18713856) |
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