|Calculated MW||86772 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Furin/PACE. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Furin, 220.127.116.11, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme, PACE, Prohormone convertase 3, Furin, Fur, Pcsk3|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Furin/PACE Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif.|
|Cellular Location||Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Cell membrane; Single-pass type I membrane protein. Note=Shuttles between the trans-Golgi network and the cell surface. Propeptide cleavage is a prerequisite for exit of furin molecules out of the endoplasmic reticulum (ER). A second cleavage within the propeptide occurs in the trans Golgi network (TGN), followed by the release of the propeptide and the activation of furin (By similarity)|
|Tissue Location||Seems to be expressed ubiquitously.|
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