|Calculated MW||81925 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of Sp3. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C|
|Other Names||Transcription factor Sp3, SPR-2, SP3|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||Sp3 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional factor that can act as an activator or repressor depending on isoform and/or post-translational modifications. Binds to GT and GC boxes promoter elements. Competes with SP1 for the GC-box promoters. Weak activator of transcription but can activate a number of genes involved in different processes such as cell-cycle regulation, hormone- induction and house-keeping.|
|Cellular Location||Nucleus. Nucleus, PML body. Note=Localizes to the nuclear periphery and in nuclear dots when sumoylated. Some localization in PML nuclear bodies|
|Tissue Location||Ubiquitously expressed.|
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