|Calculated MW||44001 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of FoxH1. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||Forkhead box protein H1, Forkhead activin signal transducer 1, Fast-1, Forkhead activin signal transducer 2, Fast-2, Foxh1, Fast1, Fast2|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||FoxH1 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).|
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Provided below are standard protocols that you may find useful for product applications.
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