|Calculated MW||73701 Da|
|Application & Usage||The peptide is used for blocking the antibody EDEM1. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||ER degradation-enhancing alpha-mannosidase-like protein 1, Edem1, Edem|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||EDEM1 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass type II membrane protein|
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