|Calculated MW||29.8 kDa|
|Other Names||Proliferating cell nuclear antigen, PCNA, Cyclin, MGC8367,|
|Source||Baculovirus (Sf9 insect cells)|
|Storage||-20°C; Sterile filtered and (5 µg/5 µl) in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA, and 20% Glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Expression of proliferating cell nuclear antigen (PCNA) or cyclin or polymerase δ auxiliary protein is elevated in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. PCNA/cyclin may act as an auxiliary protein of DNA polymerase-δ to play a fundamental role in the initiation of cell proliferation. Recombinant PCNA was expressed with a 6x His tag in sf9 insect cells and purified by proprietary chromatographic techniques.
Almendral J.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 84:1575-1579(1987).
Travali S.,et al.J. Biol. Chem. 264:7466-7472(1989).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 414:865-871(2001).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
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