|Calculated MW||8.6 Kda|
|Storage||-80°C; A solution in DMSO ( 1 mg/ml)|
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Provided below are standard protocols that you may find useful for product applications.
Prepared by the C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin. Acts as a useful and sensitive fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Km = 0.039 µM) and for Isopeptidase-T (Km = 0.17-1.4 µM), as well as for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.
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