Ubiquitin-AMC recombinant protein
Ubiquitin-Aminomethylcoumarin
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Concentration | 1 (DMSO) |
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Calculated MW | 8.6 Kda |
Other Names | Ubiquitin-Aminomethylcoumarin |
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Gene Source | Human |
Assay&Purity | ; |
Assay2&Purity2 | HPLC; ≥95% |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; A solution in DMSO ( 1 mg/ml) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Prepared by the C-terminal derivatisation of ubiquitin with 7-amino-4-methylcoumarin. Acts as a useful and sensitive fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Km = 0.039 µM) and for Isopeptidase-T (Km = 0.17-1.4 µM), as well as for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.
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