APG12/ATG12 Blocking Peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9CQY1 |
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Gene ID | 67526 |
Calculated MW | 15207 Da |
Gene ID | 67526 |
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Application & Usage | The peptide is used for blocking the antibody activity of Apg12/Atg12. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C. |
Other Names | Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like, Atg12, Apg12, Apg12l |
Target/Specificity | APG12/ATG12 |
Formulation | 50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | APG12/ATG12 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Atg12 {ECO:0000312|MGI:MGI:1914776} |
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Synonyms | Apg12, Apg12l |
Function | Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. |
Cellular Location | Cytoplasm. Preautophagosomal structure membrane {ECO:0000250|UniProtKB:O94817}; Peripheral membrane protein {ECO:0000250|UniProtKB:O94817}. Note=TECPR1 recruits the ATG12- ATG5 conjugate to the autolysosomal membrane {ECO:0000250|UniProtKB:O94817} |
Tissue Location | Ubiquitous. |
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