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NEDD8-AMC recombinant protein
NEDD8-Aminomethylcoumarin
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Concentration | 1 (DMSO) |
|---|---|
| Calculated MW | 8.735 kDa |
| Other Names | NEDD8-Aminomethylcoumarin |
|---|---|
| Gene Source | Human |
| Assay&Purity | ; |
| Assay2&Purity2 | HPLC; ≥95% |
| Recombinant | Yes |
| Format | Liquid |
| Storage | -80°C; A solution in DMSO ( 1 mg/ml) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Prepared by the C-terminal derivatisation of NEDD8 with 7-amino-4-methylcoumarin. NEDD8-AMC acts as a useful and sensitive fluorogenic substrate for deNEDDylating enzymes, including NEDP1 and UCH-L3. It is a useful reagent for the study of deNEDDylating activity where detection sensitivity or continuous monitoring of activity is essential.
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