|Calculated MW||8.5 kDa|
|Other Names||Ubiquitin Aldehyde|
|Storage||-80°C. Do not lyophilize. Do not neutralize until immediately prior to use. Avoid presence of amino containing compounds.; An aqueous solution containing 0.15 M HCl|
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Provided below are standard protocols that you may find useful for product applications.
The C-terminal glycine carboxyl of Ubiquitin is synthetically modified to an aldehyde. Ubiquitin Aldehyde (Ub-H) is useful in the stabilization of ubiquitin-protein conjugates in vitro, enhancing their accumulation in cell lysates and tissue extracts. Inhibition of deubiquitinylating enzyme activity by Ub-H can be used to identify and confirm such activity and to determine the inhibition kinetics for a particular enzyme. Recommended concentration for maximal inhibition is 2-5 µM. Co-crystallization of ubiquitin aldehyde with specific deubiquitinylating enzymes (the inhibitor mimics the natural ubiquitin substrate) has also been used to probe enzyme:substrate interactions.
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