UbcH1, human recombinant protein (GST-tag)
UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme
|Calculated MW||22.4 kDa|
|Other Names||UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme|
|Storage||-80°C; Supplied at a concentration of 1.0 mg/ml in 20 mM TBS, pH 7.5 containing 1.0 mM DTT.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM.
Kalchman M.A.,et al.J. Biol. Chem. 271:19385-19394(1996).
Kikuchi J.,et al.Arterioscler. Thromb. Vasc. Biol. 20:128-134(2000).
Furukawa Y.,et al.Electrophoresis 21:338-346(2000).
Li W.B.,et al.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.