UbcH1, human recombinant protein (GST-tag)
UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P61086 |
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Concentration | 1 |
Calculated MW | 22.4 kDa |
Other Names | UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme |
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Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; Supplied at a concentration of 1.0 mg/ml in 20 mM TBS, pH 7.5 containing 1.0 mM DTT. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM.
References
Kalchman M.A.,et al.J. Biol. Chem. 271:19385-19394(1996).
Kikuchi J.,et al.Arterioscler. Thromb. Vasc. Biol. 20:128-134(2000).
Furukawa Y.,et al.Electrophoresis 21:338-346(2000).
Li W.B.,et al.Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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