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UbcH5a, human recombinant protein (His-tag)

UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme

Product info
Primary Accession P51668
Concentration 1
Calculated MW 17.0 kDa
Additional Info
Gene ID 7321
Gene Symbol UB2D1
Other Names UbcH5a, Ubiquitin conjugating enzyme H5a, Ubiquitin conjugating enzyme, Ubiquitin-conjugating enzyme E2 D1, Stimulator of Fe transport, UBC4/5 homolog, UbcH5, Ubiquitin carrier protein D1, Ubiquitin-conjugating enzyme E2(17)KB 1, Ubiquitin-conjugating enzyme E2-17 kDa 1, Ubiquitin-protein ligase D1
Gene Source Human
Source E. coli
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 HPLC;
Recombinant Yes
Format Liquid
Storage -80°C; Supplied as a liquid in 20 mM Tris-HCl, pH 8, containing 0.5 mM DTT.
Citations (0)

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UbcH5a is highly homologous to the S. cerevisiae Ubc4/5 family of E2 enzymes demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5a, together with UbcH5b and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors. Specifically, UbcH5a stimulates the conjugation of ubiquitin to the tumor suppressor p532. Useful for in vitro ubiquitinylation reactions. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM depending upon conditions.


Scheffner M.,et al.Proc. Natl. Acad. Sci. U.S.A. 91:8797-8801(1994).
Gehrke S.G.,et al.Blood 101:3288-3293(2003).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Deloukas P.,et al.Nature 429:375-381(2004).

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$ 350.00
$ 145.00
Cat# PBV10441r
(40 western blots)
Availability: 5-7days
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