Heat Shock Protein 90 (Hsp90 beta) NT-His Tag, human recombinant protein
Heat Shock Protein 90, HSP90
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P08238 |
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Concentration | 5 |
Calculated MW | 83.4 kDa |
Gene ID | 3326 |
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Gene Symbol | HSP90AB1 |
Other Names | Heat Shock Protein 90, HSP90 |
Gene Source | Human |
Source | Sf9 cells |
Assay&Purity | SDS-PAGE; ≥99% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | MGSSHHHHHHSS GLVPRGSHMPEE VHHGEEEVETFP EEVHHGEEEVET FAFQAEIAQLMSL IINTFYSNKEIFLRE LISNASDALDKIRY ESLTDPSKLDSGK ELKIDIIPNPQERTL TLVDTGIGMTKAD LINNLGTIAKSGTK AFMEALQAGADIS MIGQFGVGFYSA YLVAEKVVVITKH NDDEQYAWESS AGGSFTVRADHG EPIGRGTKVILHL KEDQTEYLEERR VKEVVKKHSQFI GYPITLYLEKERE KEISDDEAEEEK GEKEEEDKDDEE KPKIEDVGSDEE DDSGKDKKKKTK KIKEKYIDQEELN KTKPIWTRNPDDI TQEEYGEFYKSL TNDWEDHLAVK HFSVEGQLEFRA LLFIPRRAPFDLF ENKKKKNNIKLY VRRVFIMDSCDE LIPEYLNFIRGVV DSEDLPLNISRE MLQQSKILKVIRK NIVKKCLELFSEL AEDKENYKKFYE AFSKNLKLGIHED STNRRRLSELLR YHTSQSGDEMT SLSEYVSRMKET QKSIYYITGESKE QVANSAFVERV RKRGFEVVYMT EPIDEYCVQQLK EFDGKSLVSVTK EGLELPEDEEEK KKMEESKAKFE NLCKLMKEILDKK VEKVTISNRLVSS PCCIVTSTYGWT ANMERIMKAQAL RDNSTMGYMMA KKHLEINPDHPIVE TLRQKAEADKNDK AVKDLVVLLFETALL SSGFSLEDPQTH SNRIYRMIKLGLG IDEDEVAAEE PNAAVPDEIPPLEG DEDASRMEEVD |
Format | Liquid |
Storage | -80°C; 5mg/ml in 40 mM HEPES/KOH, pH 7.5, containing 20 mM potassium chloride and 5% glycerol |
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Provided below are standard protocols that you may find useful for product applications.
Background
Hsp90 is a molecular chaperone and is one of the most abundant proteins in unstressed cells. In mammalian cells, there are two genes encoding cytosolic Hsp90 homologues, with the human Hsp90α showing 85% sequence identity to Hsp90β. The recombinant Hsp90β produced from Sf9 cells represents the full length (a.a. 1-723) of Hsp90β subunit and a N-term His-tag.
References
Rebbe N.F.,et al.Gene 53:235-245(1987).
Rebbe N.F.,et al.J. Biol. Chem. 264:15006-15011(1989).
Hoffmann T.,et al.Gene 74:491-501(1988).
Lu L.,et al.Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
Wiemann S.,et al.Genome Res. 11:422-435(2001).
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