GDF9, human recombinant protein
Growth/differentiation factor 9
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O60383 |
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Calculated MW | 31.2 kDa |
Gene ID | 2661 |
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Gene Symbol | GDF-9 |
Other Names | Growth/differentiation factor 9 |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥98% |
Assay2&Purity2 | HPLC; ≥98% |
Recombinant | Yes |
Application Notes | Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers |
Format | Lyophilized protein |
Storage | -20°C; Sterile filtered and lyophilized with no additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
GDF-9 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-3. Unlike most TGF-family members, GDF-9 and GDF-3 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. GDF-9 is expressed in oocytes and is required for normal ovarian folliculogenesis. Recombinant human GDF-9 is a 31.0 kDa non-disulfide-linked homodimer containing two 135 amino acid polypeptide chains with 6XHis on its N-terminus.
References
Schmutz J.,et al.Nature 431:268-274(2004).
Aaltonen J.,et al.J. Clin. Endocrinol. Metab. 84:2744-2750(1999).
Yamamoto N.,et al.J. Clin. Endocrinol. Metab. 87:2849-2856(2002).
Yamamoto N.,et al.J. Clin. Endocrinol. Metab. 87:4495-4495(2002).
McMahon H.E.,et al.Endocrinology 149:812-817(2008).
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