|Calculated MW||31.2 kDa|
|Other Names||Growth/differentiation factor 9|
|Application Notes||Reconstitute in H₂O to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers|
|Storage||-20°C; Sterile filtered and lyophilized with no additives|
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Provided below are standard protocols that you may find useful for product applications.
GDF-9 is a member of the TGF-β superfamily of growth and differentiation factors, and is highly homologous to GDF-3. Unlike most TGF-family members, GDF-9 and GDF-3 are not disulfide-linked dimers. GDF-3 is expressed in adult bone marrow, spleen, thymus, and adipose tissue. GDF-9 is expressed in oocytes and is required for normal ovarian folliculogenesis. Recombinant human GDF-9 is a 31.0 kDa non-disulfide-linked homodimer containing two 135 amino acid polypeptide chains with 6XHis on its N-terminus.
Schmutz J.,et al.Nature 431:268-274(2004).
Aaltonen J.,et al.J. Clin. Endocrinol. Metab. 84:2744-2750(1999).
Yamamoto N.,et al.J. Clin. Endocrinol. Metab. 87:2849-2856(2002).
Yamamoto N.,et al.J. Clin. Endocrinol. Metab. 87:4495-4495(2002).
McMahon H.E.,et al.Endocrinology 149:812-817(2008).
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