|Calculated MW||9 kDa|
|Other Names||Complement Fragments C5a, C5a anaphylatoxin chemotactic receptor|
|Application Notes||Reconstitute to a concentration of 1.0 mg/ml in ice-cold H₂O containing 2.5 mg/ml BSA. The solution should be aliquoted and immediately stored at -70°C.|
|Storage||-20°C; Sterile filtered and lyophilized with no additives|
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Provided below are standard protocols that you may find useful for product applications.
Mouse complement C5a is a 77-amino acid peptide generated during complement activation from the α-chain of complement C5. Mouse C5a shares 60% and 82% amino acid sequence identity to human and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (GPCR) (C5aR/CD88), inducing neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells. The 9 kDa mouse C5a protein expressed in E. coli contains a N-terminal His-Tag sequence.
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