|Calculated MW||30 kDa|
|Other Names||Acrp-30, GBP-28, Apm-1, 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein|
|Application Notes||Recontitute in PBS containing at least 0.1% BSA to a concentration of 0.1-1.0 mg/ml. This stock solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.|
|Storage||-20°C; Sterile filtered and lyophilized from a 0.2 µm filtered solution in 25 mM Tris, 0.15 M NaCl, pH 7.4 containing 50 µg of bovine serum albumin (BSA) per 1 µg of adiponectin.|
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Provided below are standard protocols that you may find useful for product applications.
Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and it secretion is stimulated by insulin. Mouse adiponectin shares about 83% amino acid identity with that of human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.
Scherer P.E.,et al.J. Biol. Chem. 270:26746-26749(1995).
Hu E.,et al.J. Biol. Chem. 271:10697-10703(1996).
Das K.,et al.Biochem. Biophys. Res. Commun. 280:1120-1129(2001).
Wang S.F.,et al.Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
Carninci P.,et al.Science 309:1559-1563(2005).
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