Nesfatin-1, rat recombinant protein
Nesfatin 1
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9JI85 |
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Calculated MW | 8.0 kDa |
Gene ID | 59295 |
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Gene Symbol | Nucb3 |
Other Names | Nesfatin 1, Nucleobindin-2, DNA-binding protein NEFA, Prepronefastin |
Gene Source | Rat |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Application Notes | Reconstitute with sterile H₂O to a concentration of 0.1-1 mg/ml. Further dilutions should be made with medium containing 0.2% BSA. |
Format | Lyophilized protein |
Storage | -20°C; Sterile filtered and lyophilized from PBS |
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Provided below are standard protocols that you may find useful for product applications.
Background
Nesfatin-1, a polypeptide produced by cleavage of the NEFA/nucleobindin-2 (NUCB2), is a secreted protein, expressed in the appetite-control hypothalamic nuclei. Nesfatin is involved in the regulation of the feeding behavior. Intracerebroventicular injection of nesfatin-1 reduces body weight in rats be affecting the animal food intake.
References
Hirschfeld G.,et al.Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
Hauge X.,et al.Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
Oh-I S.,et al.Nature 443:709-712(2006).
Yamawaki H.,et al.Biochem. Biophys. Res. Commun. 418:676-681(2012).
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