LBP, human recombinant protein
Lipopolysaccharide-binding protein, LBP, LBP human recombinant protein, LBP human, human LBP
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P18428 |
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Calculated MW | 58.0 kDa |
Gene ID | 3929 |
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Gene Symbol | LBP |
Other Names | Lipopolysaccharide-binding protein, LBP, LBP human recombinant protein, LBP human, human LBP |
Gene Source | Human |
Source | CHO cells |
Assay&Purity | SDS-PAGE; 90-95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Application Notes | Reconstitute in 40 µl of ultra-pure distilled H₂O. This solution can then be diluted into other aqueous buffers and stored at –20°C or –70°C for future use. |
Format | Lyophilized protein |
Storage | -20°C; Lyophilized from a concentrated protein solution (0.25 mg/ml) containing PBS, pH 7.1 |
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Provided below are standard protocols that you may find useful for product applications.
Background
Natural LBP is a 58 kDa glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophils and granulocytes. This is a His-tagged protein.
References
Schumann R.R.,et al.Science 249:1429-1431(1990).
Wilde C.G.,et al.J. Biol. Chem. 269:17411-17416(1994).
Hubacek J.A.,et al.Biochem. Biophys. Res. Commun. 236:427-430(1997).
Kirschning C.J.,et al.Genomics 46:416-425(1997).
Long J.Y.,et al.Sheng Wu Hua Xue Yu Sheng Wu Wu Li Jin Zhan 25:469-471(1998).
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