|Calculated MW||64753 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of EDEM2. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||ER degradation-enhancing alpha-mannosidase-like protein 2, EDEM2, C20orf31, C20orf49|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||EDEM2 Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Initiates the endoplasmic reticulum-associated degradation (ERAD) that targets misfolded glycoproteins for degradation in an N-glycan-dependent manner. Catalyzes the first mannose trimming step, from Man9GlcNAc2 to Man8GlcNAc2 (PubMed:25092655). Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle.|
|Cellular Location||Secreted. Endoplasmic reticulum lumen.|
|Tissue Location||Expressed ubiquitously in all tissues tested with slightly higher levels detected in small intestine and peripheral blood leukocytes and weakest levels in brain and skeletal muscle.|
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