|Calculated MW||186456 Da|
|Application & Usage||The peptide is used for blocking the antibody activity of SPHKAP. It usually blocks the antibody activity completely in Western blot analysis by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C.|
|Other Names||A-kinase anchor protein SPHKAP, SPHK1-interactor and AKAP domain-containing protein, Sphingosine kinase type 1-interacting protein, SPHKAP, KIAA1678, SKIP|
|Formulation||50 µg (0.5 mg/ml) in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA and 0.02% thimerosal.|
|Reconstitution & Storage||-20 °C|
|Precautions||SPHKAP Blocking Peptide is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.|
|Cellular Location||Cytoplasm. Note=Colocalizes with SPHK1 in the cytoplasm|
|Tissue Location||Highly expressed in heart. Both isoforms abundantly expressed in ventricle. Also expressed in spleen, ovary and brain.|
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