Epigen (EPGN), human recombinant protein
EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.
|Calculated MW||7.9 kDa|
|Other Names||EPG, Epigen, PRO9904, ALGV3072, FLJ75542, EPGN, Epithelial mitogen.|
|Application Notes||Reconstitute in sterile dH₂O not less than 100 µg/ml.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).|
|Storage||-20°C; Sterile filtered and lyophilized from 20 mM PBS buffer pH-7.4 containing 130 mM sodium chloride|
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Provided below are standard protocols that you may find useful for product applications.
EPGN is an EGF-related polypeptide growth factor that signals through the ErbB receptor-1. EPGN is produced in numerous tissues, including the testis, liver, heart and in certain tumor cells. EPGN is mitogenic for fibroblasts and epithelial cells. Human EPGN is originally synthesized as a glycosylated 14.7 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a mature soluble sequence. Recombinant Human epigen produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 7.9 kDa. Epigen is purified by proprietary chromatographic techniques.
Johnstone L.S.,et al.Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
Clark H.F.,et al.Genome Res. 13:2265-2270(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zhang Z.,et al.Protein Sci. 13:2819-2824(2004).
Kochupurakkal B.S.,et al.J. Biol. Chem. 280:8503-8512(2005).
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