Red Fluorescent Protein - R-PE (R-Phycoerythrin) recombinant protein
RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin
|Calculated MW||240 kDa|
|Other Names||RFP, Red Fluorescent Protein, R-PE, R-Phycoerythrin|
|Gene Source||Red algae|
|Source||Febico’s proprietary red algae|
|Assay&Purity||Absorbance; A566/A280 ≥ 5.5|
|Assay2&Purity2||Absorbance; A566/A498 ≤1.5|
|Application Notes||Before use, centrifuge the R-PE suspension at 10,000g for 10 min at 4C. Discard the supernatant and resuspend the pellet (R-PE) into the desired buffer. R-PE is stored in a salt solution and it must be extensively dialyzed (Can use BioVision’s Dialyzer tubes) or desalted in columns prior to use, to remove any remaining ammonium sulphate.|
|Storage||2-8°C ; R-PE is supplied in 100 mM Potassium Phosphate buffer, pH 7.0 with 60% Saturated (NH4)2SO4, 1 mM EDTA and 1 mM Sodium Azide.|
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Provided below are standard protocols that you may find useful for product applications.
R-phycoerythrin (R-PE) is an intensely bright phycobiliprotein isolated from red algae that exhibits extremely bright red-orange fluorescence with high quantum yields. The broad excitation spectrum provides the advantage for multi-color immunofluorescent staining or cell sorting. R-PE consists of a, b and g subunits and is present as (ab) 6g. R-PE and the closely related BPE are the most intensely fluorescent phycobiliproteins having orange fluorescence. R-PE is a large molecule used for fluorescence-based detection, primarily in flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity but not photostability. In practical applications, the sensitivity of R-PE conjugates is usually 5 to 10 times greater than those of the corresponding fluorescein conjugates.
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