Human recombinant protein PTP1B
Human Recombinant PTP1B
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P18031 |
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Concentration | 1 |
Calculated MW | 37.3 kDa (321 amino acids) |
Gene ID | 5770 |
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Gene Symbol | PTPN1 |
Other Names | Protein tyrosine phosphatase, non-receptor type 1, PTPN1, Protein-tyrosine phosphatase 1B, Human Recombinant PTP1B, |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Sequence | MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML RVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHEDLE PPPEHIPPPP RPPKRILEPH N |
Format | Liquid |
Storage | -80°C; 1 mg/ml solution in 25 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 2 mM β mercaptoethanol, 1 mM EDTA, 20% Glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein coding region of the catalytic domain of PTP-1B (amino acids 1-321) was cloned into an E. coli expression vector. The catalytic domain of PTP-1B was overexpressed in E. coli as a soluble protein, and it was purified by conventional column chromatographic techniques.
References
Chernoff J.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:2735-2739(1990).
Brown-Shimer S.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:5148-5152(1990).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
Deloukas P.,et al.Nature 414:865-871(2001).
Charbonneau H.,et al.Proc. Natl. Acad. Sci. U.S.A. 86:5252-5256(1989).
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