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Human recombinant protein PP2C alpha

Human Recombinant PP2C alpha

     
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Product info
Primary Accession P35813
Concentration 1
Calculated MW 46.6 kDa (418aa)
Additional Info
Gene ID 5494
Gene Symbol PPM1A
Other Names Protein phosphatase 1A (formerly 2C), human recombinant PP2C alpha, hrPP2C alpha, human PP2C alpha, recombinant PP2C alpha, magnesium-dependent, alpha isoform, PPM1A, PP2CA, PP2C-ALPHA.
Gene Source Human
Source E. coli
Assay&Purity SDS-PAGE; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWIL FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC MGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RALGDFDYKC VHGKGPTEQL VSPEPEVHDI ERSEEDDQFI ILACDGIWDV MGNEELCDFV RSRLEVTDDL EKVCNEVVDT CLYKGSRDNM SVILICFPNA PKVSPEAVKK EAELDKYLEC RVEEIIKKQG EGVPDLVHVM RTLASENIPS LPPGGELASK RNVIEAVYNR LNPYKNDDTD STSTDDMW
Format Liquid
Storage -80°C; 1 mg/ml solution in 10 mM Tris-HCl, pH 7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20% glycerol.
Citations (0)

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Background

Protein phosphatase 2C (PP2C alpha) is a Mn2+- or Mg2+- dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2C alpha (amino acids 1-382) was cloned into an E. coli expression vector. PP2C alpha was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.

References

Mann D.J.,et al.Biochim. Biophys. Acta 1130:100-104(1992).
Takekawa M.,et al.EMBO J. 17:4744-4752(1998).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Goshima N.,et al.Nat. Methods 5:1011-1017(2008).
Heilig R.,et al.Nature 421:601-607(2003).

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$ 270.00
Cat# PBV10542r
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