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E. Coli recombinant protein SlyD
E. Coli Recombinant SlyD
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P0A9K9 |
|---|---|
| Concentration | 1 |
| Calculated MW | 21 kDa (196 aa, 1-196 aa) |
| Gene ID | 12931688 |
|---|---|
| Gene Symbol | SLYD |
| Other Names | FKBP-type peptidyl-prolyl cis-trans isomerase. |
| Gene Source | E. Coli |
| Source | E. Coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | >220 nmoles/min/mg |
| Sequence | MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH |
| Format | Liquid |
| Storage | -80°C; 1 mg/ml solution in 20 mM Tris-HCl (pH 7.5). |
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Provided below are standard protocols that you may find useful for product applications.
Background
SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
References
Wuelfing C.,et al.J. Biol. Chem. 269:2895-2901(1994).
Roof W.D.,et al.J. Biol. Chem. 269:2902-2910(1994).
Horne S.M.,et al.Arch. Microbiol. 163:357-365(1995).
Blattner F.R.,et al.Science 277:1453-1462(1997).
Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006).
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