Human recombinant protein PPP1CA
Human Recombinant PPP1CA
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P62136 |
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Concentration | 0.25 |
Calculated MW | 39.7 kDa (350 aa, 1-330 aa + NT His-Tag) |
Gene ID | 5499 |
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Gene Symbol | PPP1CA |
Other Names | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PP-1A, PP1alpha, PPP1A. |
Gene Source | Human |
Source | E. Coli |
Assay&Purity | SDS-PAGE; ≥80% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | >3,000 units/mg |
Sequence | MGSSHHHHHH SSGLVPRGSH MSDSEKLNLD SIIGRLLEVQ GSRPGKNVQL TENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVQGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAD KNKGKYGQFS GLNPGGRPIT PPRNSAKAKK |
Format | Liquid |
Storage | -80°C; 0.25 mg/ml solution in 50 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 1 mM DTT, 0.15 M NaCl, 1 mM MnCl2 and 0.1 mM PMSF. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Serine/Threonine-protein phosphatase PP1-alpha catalytic subunit (PPP1CA) is crucial for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. The PPP1CA protein may have a significant role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.
References
Song Q.,et al.Gene 129:291-295(1993).
Durfee T.,et al.Genes Dev. 7:555-569(1993).
Tung L.,et al.Submitted (APR-1991) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
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