|Calculated MW||59.2 kDa (549 aa, 47-574 aa + NT His-Tag)|
|Other Names||Pyruvate kinase isozyme R/L, PK1, PKL, PKR, PKRL, RPK.|
|Sequence||MGSSHHHHHH SSGLVPRGSH MLTQELGTAF FQQQQLPAAM ADTFLEHLCL LDIDSEPVAA RSTSIIATIG PASRSVERLK EMIKAGMNIA RLNFSHGSHE YHAESIANVR EAVESFAGSP LSYRPVAIAL DTKGPEIRTG ILQGGPESEV ELVKGSQVLV TVDPAFRTRG NANTVWVDYP NIVRVVPVGG RIYIDDGLIS LVVQKIGPEG LVTQVENGGV LGSRKGVNLP GAQVDLPGLS EQDVRDLRFG VEHGVDIVFA SFVRKASDVA AVRAALGPEG HGIKIISKIE NHEGVKRFDE ILEVSDGIMV ARGDLGIEIP AEKVFLAQKM MIGRCNLAGK PVVCATQMLE SMITKPRPTR AETSDVANAV LDGADCIMLS GETAKGNFPV EAVKMQHAIA REAEAAVYHR QLFEELRRAA PLSRDPTEVT AIGAVEAAFK CCAAAIIVLT TTGRSAQLLS RYRPRAAVIA VTRSAQAARQ VHLCRGVFPL LYREPPEAIW ADDVDRRVQF GIESGKLRGF LRVGDLVIVV TGWRPGSGYT NIMRVLSIS|
|Storage||-80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 1 mM DTT and 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
PKLR is a pyruvate kinase which catalyzes the trans phosphorylation of Phosphoenolpyruvate into pyruvate and ATP. That is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. The L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.
Kanno H.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:8218-8221(1991).
Tani K.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:1792-1795(1988).
Kanno H.,et al.Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
Kanno H.,et al.Biochem. Biophys. Res. Commun. 188:516-523(1992).
Tani K.,et al.Biochem. Biophys. Res. Commun. 143:431-438(1987).
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