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Human recombinant protein DHFR
Human Recombinant DHFR
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P00374 |
|---|---|
| Concentration | 1 |
| Calculated MW | 23.6 kDa (207 aa, 1-187 aa + NT His-Tag) |
| Gene ID | 1719 |
|---|---|
| Gene Symbol | DHFR |
| Other Names | Dihydrofolate reductase. |
| Gene Source | Human |
| Source | E. Coli |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Results | 1.5 - 2.5 units/ml |
| Sequence | MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND |
| Format | Liquid |
| Storage | -80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 30% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Human dihydrofolate reductase has been used in a study to investigate the stable expression of green fluorescent protein and the targeted disruption of thioredoxin peroxidase-1 gene in Babesia bovis. Human dihydrofolate reductase has also been used in a study to investigate the structural analysis of human dihydrofolate reductase as a binary complex.
References
Chen M.-J.,et al.J. Biol. Chem. 259:3933-3943(1984).
Masters J.N.,et al.Gene 21:59-63(1983).
Yang J.K.,et al.J. Mol. Biol. 176:169-187(1984).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Schmutz J.,et al.Nature 431:268-274(2004).
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