|Calculated MW||18.8 kDa (176 aa, 1-156 aa + NT His Tag)|
|Other Names||Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 isoform 2, Parvulin-14, Parvulin-17, PPIase Pin4, hEPVH, PAR14, PAR17|
|Sequence||MGSSHHHHHH SSGLVPRGSH MPMAGLLKGL VRQLERFSVQ QQASKMPPKG KSGSGKAGKG GAASGSDSAD KKAQGPKGGG NAVKVRHILC EKHGKIMEAM EKLKSGMRFN EVAAQYSEDK ARQGGDLGWM TRGSMVGPFQ EAAFALPVSG MDKPVFTDPP VKTKFGYHII MVEGRK|
|Storage||-80°C; 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 mM PMSF, 1 mM DTT and 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (PIN4) is a peptidyl-prolyl cis/trans isomerase (PPIase) which interacts with NIMA and is vital for cell cycle regulation. PIN4 has 2 different isoforms: PAR14 and PAR17. Furthermore, Pin4 protein binds to double-stranded DNA under physiological salt conditions.
Rulten S.L.,et al.Biochem. Biophys. Res. Commun. 259:557-562(1999).
Uchida T.,et al.FEBS Lett. 446:278-282(1999).
Ross M.T.,et al.Nature 434:325-337(2005).
Mueller J.W.,et al.BMC Mol. Biol. 7:9-9(2006).
Kessler D.,et al.BMC Biol. 5:37-37(2007).
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