|Calculated MW||11.142 kDa|
|Storage||-80°C; Lyophilized powder|
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
hSUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.
Lapenta V.,et al.Genomics 40:362-367(1997).
Boddy M.N.,et al.Oncogene 13:971-982(1996).
Shen Z.,et al.Genomics 36:271-279(1996).
Mahajan R.,et al.Cell 88:97-107(1997).
Matunis M.J.,et al.J. Cell Biol. 135:1457-1470(1996).
If you have any additional inquiries please email technical services at email@example.com.