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recombinant protein hSUMO1-AMC

Recombinant hSUMO1-AMC

     
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Product info
Primary Accession P63165
Calculated MW 11.142 kDa
Additional Info
Gene ID 7341
Gene Symbol SUMO1
Gene Source Human
Assay&Purity RP-HPLC; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Format Lyophilized powder
Storage -80°C; Lyophilized powder
Citations (0)

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Background

hSUMO1-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO1. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO1-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.

References

Lapenta V.,et al.Genomics 40:362-367(1997).
Boddy M.N.,et al.Oncogene 13:971-982(1996).
Shen Z.,et al.Genomics 36:271-279(1996).
Mahajan R.,et al.Cell 88:97-107(1997).
Matunis M.J.,et al.J. Cell Biol. 135:1457-1470(1996).

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$ 455.00
Cat# PBV10645r-50
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