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recombinant protein hSUMO2-AMC

Recombinant hSUMO2-AMC

     
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Product info
Primary Accession P61956
Calculated MW 10.619 kDa
Additional Info
Gene ID 6613
Gene Symbol SUMO2
Gene Source Human
Assay&Purity RP-HPLC; ≥95%
Assay2&Purity2 N/A;
Recombinant Yes
Format Lyophilized powder
Storage -80°C; Lyophilized powder
Citations (0)

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Background

hSUMO2-AMC is prepared via the conjugation of 7-amino-4-methylcoumarin (AMC) to the C-terminal Gly of hSUMO2. This conjugation quenches the intrinsic fluorescence of AMC. Upon incubation with a protease recognizing hSUMOs, such as Senp1 or Senp2, AMC is released and the increase in fluorescence at 460 nm (Exc. 380 nm) can be measured. The enhanced purity of hSUMO2-AMC compared to that of other commercial vendors leads to a greater signal to noise ratio. This protein contains no extraneous tags.

References

Mannen H.,et al.Biochem. Biophys. Res. Commun. 222:178-180(1996).
Lapenta V.,et al.Genomics 40:362-367(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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$ 455.00
Cat# PBV10646r-50
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