|Calculated MW||25.656 kDa (Band migrates faster on gels)|
|Assay&Purity||Western Blot; ≥95%|
|Storage||-80°C; 2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Increasingly, researchers are focusing on the role poly-ubiquitin chains linked through K11. This post-translational modification has been linked to the ERAD cycle as a signal, similar to K48 linkage, for proteasomal degradation. More recently, K11 linkage appears to play an important role in cell cycle signaling, as it is associated with the anaphase promoting complex (APC) of ubiquitination machinery. These tri-ubiquitin chains are generated from the enzymatic linkage (UBE2S) of wild-type ubiquitin through lysine 11 and purified to >95% homogeneity by ion exchange chromatography.
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