Human recombinant protein Ubiquitin Activating Enzyme E1
Human recombinant Ubiquitin Activating Enzyme E1
|Calculated MW||118 kDa|
|Other Names||UBE1, UBA1, POC20, A1S9T, A1ST, AMCX1, GXP1, SMAX2|
|Storage||-80°C; In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate.
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Handley P.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:258-262(1991).
Handley P.M.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:7456-7456(1991).
Ross M.T.,et al.Nature 434:325-337(2005).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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