|Calculated MW||28.707 kDa with a His6SUMO Tag|
|Other Names||Ubiquitin-conjugating enzyme E2 D3, Ubiquitin-protein ligase D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating enzyme E2-17 kDa 3, UBC4/5, UBCH5C, MGC5416, MGC43926, UBE2D3.|
|Storage||-80°C; 75 µL of a 40 µM solution (3 nmoles) in 25 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
UBE2D3 (UbcH5b) is an E2 (ubiquitin conjugating enzyme) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E3 enzymes. The UbcH5 family (including UbcH5a and UbcH5c, having 88% and 89% sequence identity with UbcH5b respectively) are the human homologues of the Saccharomyces cerevisiae UBC4/5 family (~79% sequence identity). Members of the UbcH5 family have been implicated in a number of inflammatory and cancer pathways including the ubiquitylation of p53 and IκBα with the help of different E3 ligases (Hdm2 and SCF complexes respectively).
Jensen J.P.,et al.J. Biol. Chem. 270:30408-30414(1995).
Chang H.-M.,et al.Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Hillier L.W.,et al.Nature 434:724-731(2005).
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