Human recombinant protein UBE2L3
Human recombinant UBE2L3
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P68036 |
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Calculated MW | 17.861 kDa |
Gene ID | 7332 |
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Gene Symbol | UBE2L3 |
Other Names | Ubiquitin-conjugating enzyme E2 L3, EC 6.3.2.19, Ubiquitin-protein ligase L3, Ubiquitin carrier protein L3, UbcH7, E2-F1, L-UBC, UbcM4. |
Gene Source | Human |
Assay&Purity | RP-HPLC; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; 75 µL of a 40 µM solution (3 nmoles) in 50 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
UBE2L3 (UbcH7) is an E2 (ubiquitin conjugating enzyme) ligase involved in the conjugation of ubiquitin to target substrates, along with E1 and E3 enzymes. UbcH7 has been shown to work with HECT domain E3’s (E6-AP), in addition to RING containing E3’s. UbcH7 is homologous to the UbcH5 family.
References
Robinson P.A.,et al.Mamm. Genome 6:725-731(1995).
Nuber U.,et al.J. Biol. Chem. 271:2795-2800(1996).
Moynihan T.P.,et al.Genomics 51:124-127(1998).
Poloumienko A.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Collins J.E.,et al.Genome Biol. 5:R84.1-R84.11(2004).
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