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Yeast recombinant protein Ubc13 (UBE2N)
Yeast recombinant Ubc13 (UBE2N)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P52490 |
|---|---|
| Calculated MW | 18.462 kDa with His Tag. |
| Gene ID | 851666 |
|---|---|
| Gene Symbol | UBC13 |
| Other Names | Ubiquitin-conjugating enzyme E2 N, Bendless-like ubiquitin-conjugating enzyme, Ubiquitin carrier protein N, Ubiquitin-protein ligase N, BLU, MGC8489, UbcH-ben, MGC131857. |
| Gene Source | Yeast |
| Source | Saccharomyces cerevisiae |
| Assay&Purity | SDS-PAGE; ≥95% |
| Assay2&Purity2 | N/A; |
| Recombinant | Yes |
| Format | Liquid |
| Storage | -80°C; 75 µL of a 40 µM solution (3 nmoles) in 50 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubc13 is an E2 (ubiquitin conjugating enzyme) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E3 enzymes. Ubc13 acts with the UEV protein Mms2 forming a heterodimeric complex that assembles K63-linked polyubiquitin chains utilized in nondegradative ubiquitin signaling pathways (DNA repair, signal transduction). The Ubc13/Mms2 complex is unique in that it has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3. UBE2N mediates the transcription of some target genes and is believed to have a role in cell cycle progression; cellular differentiation and DNA repair mechanisms which ensure cell survival after DNA damage.
References
Matuschewski K.,et al.J. Biol. Chem. 271:2789-2794(1996).
Jacq C.,et al.Nature 387:75-78(1997).
Engel S.R.,et al.G3 (Bethesda) 4:389-398(2014).
Ghaemmaghami S.,et al.Nature 425:737-741(2003).
Starita L.M.,et al.Proteomics 12:236-240(2012).
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