|Calculated MW||15.544 kDa with His Tag.|
|Storage||-80°C; 75 µL of a 40 µM solution (3 nmoles) in 50 mM Tris, pH 7.5, 150 mM NaCl, 10 mM DTT and 10% glycerol.|
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Provided below are standard protocols that you may find useful for product applications.
Mms2 is a UEV (ubiquitin E2 variant) protein that acts with the E2 enzyme Ubc13 in a heterodimeric complex which assembles K63-linked polyubiquitin chains utilized in non-degradative ubiquitin signaling pathways (DNA repair, signal transduction). The UEV proteins are structurally similar to E2 enzymes displaying the same fold, but lacking the active site cysteine of the E2. The Ubc13/Mms2 complex is unique in that it is has the ability to form free K63-linked polyubiquitin chains in solution in the absence of an E3.
Broomfield S.,et al.Proc. Natl. Acad. Sci. U.S.A. 95:5678-5683(1998).
Rieger M.,et al.Yeast 13:1077-1090(1997).
Tettelin H.,et al.Nature 387:81-84(1997).
Engel S.R.,et al.G3 (Bethesda) 4:389-398(2014).
Xiao W.,et al.Nucleic Acids Res. 26:3908-3914(1998).
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