Human recombinant protein UBE2E3 (UbcH9)
Human recombinant UBE2E3 (UbcH9)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q969T4 |
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Calculated MW | 22.913 kDa |
Gene ID | 10477 |
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Gene Symbol | UBE2E3 |
Other Names | Ubiquitin-conjugating enzyme E2E 3, Ubiquitin-conjugating enzyme E2-23 kDa, UBCH9, Ubiquitin-protein ligase E3, Ubiquitin-conjugating enzyme E2E3. |
Gene Source | Human |
Assay&Purity | RP-HPLC; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; 75 µL of a 40 µM solution (3 nmoles) in 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT and 10% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin-conjugating enzyme E2 E3 (UBE2E3 alias UbcH9) is a protein that in humans is encoded by the UBE2E3 gene. The deduced 207-amino acid protein shares over 94% amino acid identity with the UBC domains of class III E2s, UbcH6, UBE2E2, UbcM2, UbcM3, and UbcD2. But the N-terminal extension exhibited little homology among these, except for UbcM2, which showed 100% identity, and which is thought to be a mouse counterpart. The UBE2E3 form a thioester bond with ubiquitin (Ub) in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase.
References
Ito K.,et al.Cytogenet. Cell Genet. 84:99-104(1999).
Shen Y.,et al.Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
Xin Y.R.,et al.Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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