Human CellExp SCF, Human recombinant protein
Human Cellexp Human Recombinant SCF
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P21583 |
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Calculated MW | 35 to 45 kDa, monomer, glycosylated |
Gene ID | 4254 |
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Gene Symbol | KITLG |
Other Names | Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kit l, DKFZp686F2250. |
Gene Source | Human |
Source | Human 293 cell expressed |
Assay&Purity | SDS-PAGE; > 95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Results | 5 to 25 ng/ml |
Application Notes | Reconstitute in sterile PBS containing 0.1% endotoxin-free recombinant human serum albumin. |
Format | Lyophilized |
Storage | -80°C; Lyophilized in PBS. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms; cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).
References
Martin F.H.,et al.Cell 63:203-211(1990).
Anderson D.M.,et al.Cell Growth Differ. 2:373-378(1991).
Blair H.C.,et al.Biochem. Biophys. Res. Commun. 255:778-784(1999).
Han C.,et al.Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
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