Protein A (Liquid form) recombinant protein
Protein A
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | YP_498670 |
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Concentration | 50 |
Calculated MW | 39.21 kDa |
Gene ID | 3919448 |
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Other Names | Protein A |
Gene Source | Staphylococcus aureus |
Source | E. coli |
Assay&Purity | SDS-PAGE; ≥99% |
Assay2&Purity2 | HPLC; ≥99% |
Recombinant | Yes |
Format | Liquid |
Storage | -20°C; 50 mg/ml in H₂O |
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Provided below are standard protocols that you may find useful for product applications.
Background
The recombinant Protein A is a genetically engineered protein containing five Ig-binding regions of protein A and 6x His-tag on N-terminus. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the recombinant Protein A to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3). It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
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