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Protein A/G-FITC recombinant protein
Protein A/G
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Calculated MW | 59.7 kDa |
|---|
| Other Names | Protein A/G |
|---|---|
| Source | E. coli |
| Assay&Purity | SDS-PAGE; ≥97% |
| Assay2&Purity2 | HPLC; ≥97% |
| Recombinant | Yes |
| Application Notes | Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution. |
| Format | Lyophilized |
| Storage | -20°C; Lyophilized with no additives |
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Provided below are standard protocols that you may find useful for product applications.
Background
Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody. Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. Protein A/G-FITC is prepared by conjugating recombinant Protein A/G to FITC. The conjugation was optimized to provide the maximal binding capacity for IgG and also maximum FITC fluorescence.
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