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Protein L recombinant protein

Protein L

     
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Product info
Primary Accession CAA25612
Calculated MW 43.2 kDa with 5 IgG binding sites
Additional Info
Other Names Protein L
Gene Source Peptostreptococcus Magnus
Source E. coli
Assay&Purity SDS-PAGE; ≥98%
Assay2&Purity2 HPLC;
Recombinant Yes
Application Notes Reconstitution in H₂O to a concentration of 5 mg/ml gives a clear solution.
Format Lyophilized
Storage -20°C; Lyophilized with no additives
Citations (0)
citation

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Background

Protein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein L detection using anti-His-tag antibodies. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.

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Discontinued
Cat# PBV10729r-1
Size:

Ordering Information

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