GSK-3a-GST Fusion Protein recombinant protein
GSK
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P49840 |
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Concentration | 2 |
Calculated MW | 37.0 kDa |
Gene ID | 2931 |
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Gene Symbol | GSK3A |
Other Names | GSK, Glycogen synthase kinase-3 (GSK-3) |
Gene Source | Human |
Source | E. coli |
Assay&Purity | SDS-PAGE; |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Format | Liquid |
Storage | -20°C; 2 mg/ml in storage buffer containing 20 mM Tris-HCl (pH 7.5), 50 mM NaCl, 2 mM EDTA, 1 mM DTT, and 50% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
GSK-3 is one of the Akt substrates. It can be phosphorylated by Akt at Ser21 of GSK-3α or Ser9 of GSK-3β. The phosphorylation of GSK negatively regulates its kinase activity. The recombinant GSK-3α fusion protein serves as a useful substrate for assaying Akt kinase activity.
References
He X.,et al.Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases.
Hoshino T.,et al.Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
Nikoulina S.E.,et al.Diabetes 49:263-271(2000).
Phiel C.J.,et al.Nature 423:435-439(2003).
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