IL-12p80, Human recombinant protein
NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alp
|Calculated MW||80 kDa|
|Other Names||NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.|
|Source||(BTI-Tn-5B1-4) Hi-5 Insect Cells|
|Sequence||IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.|
|Storage||-20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Acetate - pH 5.6, 90 mM NaCl, 2% Sucrose and 4.0% Mannitol.|
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Provided below are standard protocols that you may find useful for product applications.
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23, or can form a homodimer designated as IL-12 p80. Elevated levels of IL-12 p80 are correlated with macrophage recruitment and increased inflammation in asthma and respiratory viral infection models. Recombinant human IL-12 p80 is an 80.0 kDa disulfide linked homodimer consisting of two p40 chains of IL-12.
Gubler U.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:4143-4147(1991).
Wolf S.F.,et al.J. Immunol. 146:3074-3081(1991).
Huang D.,et al.Genes Immun. 1:515-520(2000).
Hongyuan J.,et al.Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
Stern A.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:6808-6812(1990).
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