sCD27 Ligand, Human recombinant protein
TNFSF7, CD70
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P26842 |
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Calculated MW | 19.2 kDa |
Gene ID | 939 |
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Gene Symbol | CD27 |
Other Names | TNFSF7, CD70 |
Gene Source | Human |
Source | CHO cells |
Assay&Purity | SDS-PAGE; ≥95% |
Assay2&Purity2 | HPLC; |
Recombinant | Yes |
Sequence | HHHHHHHHPS PGGSGGQRFA QAQQQLPLES LGWDVAELQL NHTGPQQDPR LYWQGGPALG RSFLHGPELD KGQLRIHRDG IYMVHIQVTL AICSSTTASR HHPTTLAVGI CSPASRSISL LRLSFHQGCT IASQRLTPLA RGDTLCTNLT GTLLPSRNTD ETFFGVQWVR P |
Target/Specificity | sCD27 |
Application Notes | Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C. |
Format | Lyophilized powder |
Storage | -20°C; Sterile filtered through a 0.2 micron filter. Lyophilized from 20 mM Sodium Citrate, pH 3.0. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. It is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell expansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the survival of CD8+ T cells, primarily by inducing increased expression of the IL-2 gene. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. BioVision’s recombinant human sCD27L contains the extracellular domain plus an N-terminal His-Tag.
References
Camerini D.,et al.J. Immunol. 147:3165-3169(1991).
Loenen W.A.,et al.J. Immunol. 149:3937-3943(1992).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).
Zhang Z.,et al.Protein Sci. 13:2819-2824(2004).
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